Omunye wemisebenzi ebalulekile lapho ukhula amazambane embewu ukuthola isivuno esihle futhi uqinisekise ikhwalithi yamazambane asezingeni elisezingeni lezidingo zokulawula. Ukufeza lezi zinhloso ekukhiqizeni imbewu yamazambane, kubalulekile ukusebenzisa izinto zomthombo wekhwalithi ephezulu. Ochwepheshe abavela egatsheni le-Federal State Budgetary Institution "Rosselkhoztsentr" e-Krasnoyarsk Territory babambe iqhaza ekukhetheni ama-clone amazambane ayisisekelo ukuze bawangenise esikweni le-vitro kanye nokuthola izinto zomthombo. Ukukhethwa kwenziwa ekutshalweni kwesizukulwane sokuqala sensimu yezinhlobo zamazambane uLady Claire, Colomba, Innovator, Argo, Lila endaweni yokulima yaseSHP Dary Malinovki LLC. Ngesikhathi sokukhethwa, ukuhlolwa okubonakalayo okuphelele kwesitshalo ngasinye okwethulwa kwenziwa. Kwakhethwa izitshalo ezazijwayeleke kakhulu ezinhlobonhlobo, ezinezici ezicacile ezichazwe kahle, ezibukeka zinempilo ngokuphelele, ezinenani leziqu eziyinhloko zezinhlobonhlobo, ezinamahlamvu anombala ofanayo, ngaphandle kwezimpawu zokugwaza, ukushwabana, ukugoqa noma ukugoqa. ukugoqa.
Isivuno sesitshalo ngasinye sambiwa ngesandla, sabekwa egodini, futhi isidleke ngasinye sahlolwa ngokwaso ukuze kutholwe ukuhlukahluka nempilo ye-tuber. Ukunakwa okukhethekile kukhokhwa ekubunjweni okujwayelekile kwezilimo eziyizigaxa zalezi zinhlobonhlobo, ukungabi khona kwezimpawu zokuma kwe-spindle, nokungabikho kwezimpawu zezifo nokukhubazeka.
Kusukela kuma-tubers akhethiwe, izicubu ezivela kuma-apical (apical) meristems atholakala engxenyeni ye-apical yezitho zemifino zizothathwa elabhorethri.
Impahla yomthombo wamazambane ikhuliswa ngaphansi kwezimo zaselabhorethri kumashubhu okuhlola, kusukela ezicutshini ze-meristematic, kusetshenziswa ubuchwepheshe be-in vitro. Ukusetshenziswa kwalobu buchwepheshe kwenza kube nokwenzeka ukuthola izinto zokutshala ezihambisana nezakhi zofuzo, ezingenalo igciwane, isikhunta kanye ne-bacterial pathogens. Abaqambi bezinhlobonhlobo kuphela abanelungelo lokutshala amazambane anjalo.
Ama-microplants okuqala e-in vitro, atholwe ngokwethula ama-clones ayisisekelo esikweni, azosatshalaliswa kumthamo odingekayo, esedlule imijikelezo eminingana yokusikwa emithonjeni yezakhi zokwenziwa.
Kamuva, ama-microplants ukuthola ama-mini-tubers atshalwa ezindaweni zokugcina izithombo, endaweni elawulwayo enokuvikelwa ezinambuzaneni. Bese kuthi ama-mini-tubers atshalwe ensimini, akhiqize isizukulwane sokuqala sensimu. Amazambane atholwe ekusakazeni kwezinto ezithuthukisiwe zomthombo (ama-mini-tubers, isizukulwane sokuqala sensimu), akhiqizwa umsunguli wezinhlobonhlobo noma umuntu ogunyazwe nguye, angabokuqala bembewu yamazambane.
Ukuthola imbewu enempilo kuyisigaba esibalulekile ekukhiqizweni kwembewu yamazambane futhi kuyisici esibalulekile esinomthelela ekukhiqizeni nasekuvunweni kwezinhlobonhlobo. Njengamanje, ukubuyiswa kwamazambane ezifweni ezibangelwa amagciwane kusetshenziswa isiko le-meristem kusetshenziswa kuwo wonke amazwe omhlaba akhiqiza amazambane. Imbewu yeqophelo eliphezulu etshalwe emapulazini embewu ayisipesheli inikeza isivuno esifinyelela ku-25-50%, uma kuqhathaniswa nembewu ewohlokayo engakaze ivuselelwe iminyaka eminingi.